In this webinar, Dr. Jihye Jo introduces PPI (protein-protein interaction) Landscape, powered by PROTEINA’s single-molecule SPID platform.
PPI Landscape transforms antibody libraries into sequence-to-affinity heatmaps, capturing 5,000 KD-precision data points per run with SPR/BLI-equivalent accuracy. Within just two weeks, the platform completes the entire workflow—from DNA library synthesis to mammalian (HEK/CHO) expression and single-molecule KD measurement—all without purification.
This quantitative Deep Mutational Scanning (qDMS) approach reveals how every mutation within the antibody CDRs affects binding affinity, guiding combinatorial library design across inter- and intra-CDR regions.
As a data-driven, high-throughput, fee-for-service solution, PPI Landscape enables rapid affinity maturation and tuning, delivering optimized antibody sequences with sub-nanomolar to picomolar affinities—at unmatched speed, scale, and precision.
Key Topics Include:
- Discover how the PPI Landscape platform transforms antibody libraries into sequence-to-affinity heatmaps using single-molecule SPID technology.
- Learn how 5,000 KD-precision data points per run enable high-throughput, quantitative Deep Mutational Scanning (qDMS) of antibody CDRs.
- Explore how inter- and intra-CDR combinatorial libraries can be rationally designed from quantitative affinity landscapes.
- Understand how this purification-free, mammalian-based workflow achieves SPR/BLI-equivalent accuracy with a two-week turnaround.
- Gain insight into applying PPI Landscape data for affinity maturation, affinity tuning, and AI-assisted antibody optimization.
Presenters
Director
High-Throughput Antibody Engineering Platform
PROTEINA
Production Partner
PROTEINA
