Lonza’s new technical note introduces a flexible, high-throughput workflow for generating and characterizing macrophage subtypes—naïve, M1, and M2—from cryopreserved PBMCs or CD14⁺ monocytes. This workflow is ideal for supporting drug discovery, immunotoxicology, and disease modeling by enabling researchers to replicate immune responses with precision.
Key features include discussion on:

• Dual Starting Materials: Discover differences between PBMCs or CD14⁺ monocytes to match your research needs.
• Validated Polarization: M1 and M2 macrophages are reliably produced using IFN-γ/LPS or IL-4/IL-10, respectively.
• Live-Cell Phagocytosis Assay: Real-time imaging quantifies functional activity across macrophage types.
• Phenotypic Confirmation: Surface markers and cytokine profiles confirm accurate polarization and This article is ideal for researchers studying immune modulation, inflammation, and therapeutic response.

Not all macrophages are created equal—Lonza helps you choose the right path.

Access the technical note: